MicroRNAs (miRNAs) Biotherapeutics
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MicroRNAs (miRNAs) are a class of small (18–24 nucleotide long) RNAs that are involved in regulation of gene expression by targeting messenger RNAs (mRNA). Deregulation of miRNAs has been reported in many human diseases such as cancer, cardiovascular disease and autoimmune disorders. Since miRNAs can be efficiently inhibited by modified/synthetic antisense oligonucleotides, there is great interest in developing anti-miRNA therapies for several diseases. In addition to tissues/cells, miRNAs were also detected in various biofluids such as serum/plasma, urine, saliva, cerebrospinal fluid and amniotic fluid. Usually miRNAs present in biofluids are packed in exosomes or associated to proteins or lipoproteins and hence protected from enzymatic degradation. Because of their stability and specificity, several studies demonstrated the utility of circulating miRNAs as diagnostic and prognostic biomarkers in disease such as cancer, cardiac disease and autoimmune disease. More recently, microRNAs have been investigated as potential safety biomarkers. Thus miRNAs received much attention not only as global intracellular and intercellular regulators but also as therapeutic targets and disease or safety biomarkers. Hence there is great interest in miRNA identification and profiling.
- Micro RNA biogenesis
- microRNA expression
- microRNA targets
- microRNA evolution
- genomics
- Application of RNA interference
- Treatment pathways
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